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PCR Troubleshooting and Optimization: The Essential Guide

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 | #3391869 in Books |  2011-01-01 | Original language:English | PDF # 1 |  9.30 x.70 x6.40l,1.30 | File type: PDF | 250 pages

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The polymerase chain reaction (PCR) is a fundamental tool in scientific research and clinical testing. Real-time PCR, combining both amplification and detection in one instrument, is a rapid and accurate method for nucleic acid detection and quantification. Although PCR is a very powerful technique, the results achieved are valid only if the appropriate controls have been employed. In addition, proper optimization of PCR conditions is required for the generation of speci...

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